Description: A Rabbit Polyclonal antibody against PDE7A from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Description: Rabbit polyclonal PDE7A antibody

Description: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Description: Unconjugated Rabbit polyclonal to PDE7A

Description: Mouse polyclonal to PDE7A

Description: A cloning plasmid for the PDE7A gene.

Description: Human PDE7A1 GenBank Accession No. NM_002603), a.a. 121-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Description: The PDE7A Assay Kit is designed_x000D_for identification of PDE7A inhibitors using fluorescence polarization. The assay is_x000D_based on the binding of a fluorescent nucleotide monophosphate generated by PDE7A_x000D_to the binding agent. The key to the PDE7A_x000D_Assay Kit is the specific binding agent. Using this kit, only two simple steps on a_x000D_microtiter plate are required for PDE7A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE7A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then_x000D_be measured using a fluorescence reader.

Description: The PDE7A Assay Kit is designed for identification of PDE7A inhibitors using_x000D_fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE7A to the binding agent. PDE7A catalyzes the hydrolysis of_x000D_the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP)._x000D_Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This_x000D_increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization_x000D_of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating_x000D_nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized_x000D_light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain_x000D_random orientations and emit light with low polarization. The key to the PDE7A Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE7A_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE7A_x000D_for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in_x000D_fluorescent polarization that can then be measured using a fluorescence reader.

Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE7A, also known as high affinity cAMP-specific phosphodiesterase 7A, has been implicated in cardiovascular function and fertility. The Rat PDE7A Assay Kit is designed for identification of inhibitors of Rat PDE7A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE7A to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The Rat PDE7A Assay Kit comes in a convenient 96-well format, with purified Rat PDE7A enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE7A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Rat PDE7A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Rat PDE7A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: The Mouse PDE7A Assay Kit is designed for identification of inhibitors of Mouse PDE7A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE7A to the binding agent._x000D_The key to the Mouse PDE7A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE7A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE7A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Mouse PDE7A (GenBank Accession No. NM_001122759), a.a. 147-482(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Description: Rat PDE7A (GenBank Accession No. NM_031080), a.a. 147-482(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Description: Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). ; N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells, inducing the expression of the luciferase reporter, whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity.

Description: Recombinant HEK293 cell line expressing rat PDE7A (phosphodiesterase 7A, accession number NM_031080). ; N-terminal FLAG-tagged rat PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line and its expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by rat PDE7A in rat PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of luciferase reporter, whereas rat PDE7A-HEK293 cells showed reduction in the level of cAMP, resulting in lowered levels of luciferase expression. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity.